《植物生理学报》 2010, 46(9): 902-908

紫外照射对葡萄果实莽草酸途径相关基因表达的影响

初英娜, 张珍珍, 潘秋红*

中国农业大学食品科学与营养工程学院, 北京100083

通信作者：潘秋红；E-mail: panqh@cau.edu.cn；Tel: 010-62736191

摘　要：

本文以花后 11 周的 ‘ 赤霞珠 ’ 葡萄果实为试材, 应用荧光实时定量 PCR 技术, 研究 6 种剂量的 UV-A、UV-B 和 UV-C 照射对莽草酸途径及后分支酸途径关键酶基因表达量的影响。结果表明, 这些基因在转录水平上对紫外诱导的响应不同步, 且有照射剂量的依赖性。一定剂量的紫外照射可显著地诱导莽草酸途径的大部分基因和后分支酸途径的VvCM-1、VvCM-2 和 VvAS 的表达; 高于或低于该剂量, 表达量明显降低。不同基因对紫外诱导的响应也存在差异: 3 种类型紫外线对莽草酸 途径入口酶的两个同源基因VvDAHPS-1 和VvDAHPS-2 的诱导效应均最为显著。随紫外波长减小, 这两个基因受诱导表达 的量有所下降。VvSK 和 VvCS 的表达只受1.2 kJ•m-2 UV-A 的诱导, 而不受 UV-B 和UV-C 照射的影响, 在后分支酸途径中紫 外对 VvCM-1 表达的诱导作用明显大于 VvCM-2 和 VvAS。

关键词：莽草酸途径; 紫外照射; 基因表达; 葡萄果实

收稿：2010-05-05   修定：2010-07-14

资助：国家自然科学基金(30971980)。

Effects of Ultraviolet Radiation on Expression of Shikimate Pathway-Related Genes in Grape (Vitis vinifera L.) Berries

CHU Ying-Na, ZHANG Zhen-Zhen, PAN Qiu-Hong*

College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China

Corresponding author: PAN Qiu-Hong; E-mail: panqh@cau.edu.cn; Tel: 010-62736191

Abstract:

The present study investigated the effects of radiation of UV-A, UV-B and UV-C, respectively, with six doses on the expression of the genes in shikimate pathway and post-chorismate pathway in grape (Vitis vinifera cv. ‘Cabernet Sauvignon’) berries at 11 weeks after full bloom, by means of real-time PCR. The results showed that the transcript abundance of these genes in response to UV radiation was dose-dependent, but not synchronized. A certain dose of UV radiation could significantly induce the expression of most of genes in shikimate pathway and the expression of VvCM-1, VvCM-2 and VvAS in post-chorismate pathway, but gene expression was reduced when higher or lower radiation was applied. Various genes showed different responses to the same type of UV radiation. Three types of UV all most significantly induced transcript abundances of both VvDAHPS-1 and VvDAHPS-2 that encode the entry isozymes of shikimate pathway, whereas short wavelength UV suppressed the accumulation of their transcripts. The expression of VvSK and VvCS was promoted only by UV-A radiation of 1.2 kJ·m^-2, but not by UV-B and UV-C radiation. In post-chorismate pathway, the transcriptional induction of VvCM-1 by UV radiation was more significant than that of either VvCM-2 or VvAS.

Key words: shikimate pathway; ultraviolet radiation; gene expression; grape berry